期刊目次

加入编委

期刊订阅

添加您的邮件地址以接收即将发行期刊数据:

Open Access Article

Journal of Modern Biotechnology Research. 2023; 1: (1) ; 6-11 ; DOI: 10.12208/j.jmbr.20230002.

Application of plant-derived extracellular vesicles in the treatment of inflammatory bowel disease
植物源细胞外囊泡在炎症性肠病治疗中的应用

作者: 蔡鹏1, 张明真2, 高博雯2 *

1 新疆莎车县 69218 部队医院 新疆喀什

2 西安交通大学基础医学院医学工程研究所 陕西西安

*通讯作者: 高博雯,单位: 西安交通大学基础医学院医学工程研究所 陕西西安;

发布时间: 2023-06-09 总浏览量: 810

摘要

炎症性肠病(inflammatory bowel disease, IBD)是一种慢性非特异性的肠道炎症性疾病。目前临床常用的化学治疗药物和免疫抑制剂会对患者产生较大的副作用,新型的免疫疗法也存在治疗效果个体差异大等问题,因此开发IBD治疗新方式具有重要意义。细胞外囊泡(EVs)是一种异构的磷脂双层封闭的生物颗粒,几乎所有的活细胞都能分泌EVs,包括植物细胞。植物源性细胞外囊泡(PDEVs)是从植物中分离出来的囊泡状纳米结构颗粒的总称。研究发现,多种PDEVs具有良好的稳定性,口服后能够穿过胃肠道的生理环境到达结肠,并且在炎症性肠病模型中显示出优良的结肠靶向性和滞留性。此外,PDEVs可作为一种脂质体实现药物递送,有望实现IBD多种药物的联合治疗。与动物源细胞外囊泡相比,低免疫原性和广泛的可获得性使得PDEVs作为治疗药物和药物载体更安全、更经济。因此,PDEVs作为一种新的治疗方法,在炎症性肠病的治疗领域展现出巨大的应用潜力。在本文中,我们回顾了目前关于PDEVs的知识,总结了PDEVs作为一种IBD治疗新方法的潜在应用。

关键词: 植物;细胞外囊泡;炎症性肠病;治疗

Abstract

Inflammatory bowel disease (IBD) is a chronic, non-specific inflammatory bowel disease. Currently, chemotherapy drugs and immunosuppressants commonly used in clinical practice may cause great side effects to patients, and new immunotherapies also have problems such as large individual differences in therapeutic effect. Therefore, it is of great significance to develop new therapeutic methods for IBD. Extracellular vesicles (EVs) are heterogeneous, bilayer sealed biological granules of phospholipids that are secreted by almost all living cells, including plant cells. Plant-derived Extracellular vesicles (PDEVs) are a general term for vesicle-like nanostructured particles isolated from plants. Multiple PDEVs have been found to be stable, able to cross the physiological environment of the gastrointestinal tract and reach the colon after oral administration, and show excellent colonic targeting and retention in inflammatory bowel disease models. In addition, PDEVs can be used as a liposome to achieve drug delivery, which is expected to realize the combination therapy of multiple drugs for IBD. Their low immunogenicity and widespread availability make PDEVs safer and more economical as therapeutic agents and drug carriers than animal-derived extracellular vesicles. Therefore, PDEVs, as a new therapeutic method, has shown great application potential in the treatment of inflammatory bowel disease. In this article, we review current knowledge about PDEVs and summarize the potential application of PDEVs as a new therapeutic approach for patients with IBD.

Key words: Plant; Extracellular Vesicles; Inflammatory Bowel Disease; Treatment

参考文献 References

[1] Axelrad, J. E., Lichtiger, S. & Yajnik, V. Inflammatory bowel disease and cancer: The role of inflammation, immunosuppression, and cancer treatment. World Journal of Gastroenterology22, 4794-4801 (2016). 

[2] Bisgaard, T. H., Allin, K. H., Keefer, L., Ananthakrishnan, A. N. & Jess, T. Depression and anxiety in inflammatory bowel disease: epidemiology, mechanisms and treatment. Nature Reviews Gastroenterology & Hepatology19, 717-726 (2022). 

[3] Goncalves, P., Araujo, J. R. & Di Santo, J. P. A Cross-Talk Between Microbiota-Derived Short-Chain Fatty Acids and the Host Mucosal Immune System Regulates Intestinal Homeostasis and Inflammatory Bowel Disease. Inflammatory Bowel Diseases24, 558-572 (2018).

[4] Yuan, D., Li, C., Huang, Q., Fu, X. & Dong, H. Current advances in the anti-inflammatory effects and mechanisms of natural polysaccharides. Critical Reviews in Food Science and Nutrition (2022). 

[5] Cui, Y., Gao, J., He, Y. & Jiang, L. Plant extracellular vesicles. Protoplasma257, 3-12 (2020). 

[6] Cieslik, M., Nazimek, K. & Bryniarski, K. Extracellular Vesicles-Oral Therapeutics of the Future. International Journal of Molecular Sciences23 (2022). 

[7] Aarts, J. et al. Flood Control: How Milk-Derived Extracellular Vesicles Can Help to Improve the Intestinal Barrier Function and Break the Gut-Joint Axis in Rheumatoid Arthritis. Frontiers in Immunology12 (2021). 

[8] Feng, J. et al. Plant-Derived Vesicle-Like Nanoparticles as Promising Biotherapeutic Tools: Present and Future. Advanced Materials (2023). 

[9] Fan, S.-J. et al. Edible plant extracellular vesicles: An emerging tool for bioactives delivery. Frontiers in Immunology13 (2022). 

[10] Zhang, M. et al. Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer. Biomaterials101, 321-340 (2016). 

[11] Zheng, L., Zheng, L., Li, B., Liu, C. C. & Pan, W. L. New technology for analysis of extracellular vesicles towards clinical diagnosis. Cancer Science112, 985-985 (2021). 

[12] Savci, Y. et al. Grapefruit-derived extracellular vesicles as a promising cell-free therapeutic tool for wound healing. Food & Function12, 5144-5156 (2021). 

[13] Stanly, C. et al. Grapefruit-Derived Micro and Nanovesicles Show Distinct Metabolome Profiles and Anticancer Activities in the A375 Human Melanoma Cell Line. Cells9 (2020). 

[14] Auger, C. et al. Extracellular Vesicle Measurements with Nanoparticle Tracking Analysis: A Different Appreciation of Up and Down Secretion. International Journal of Molecular Sciences23 (2022). 

[15] Kocholata, M., Maly, J., Martinec, J. & Malinska, H. A. U. E. R. Plant Extracellular Vesicles and Their Potential in Human Health Research, the Practical Approach. Physiological Research71, 327-339 (2022). 

[16] Tong, L. et al. Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis. Sci Adv9, eade5041 (2023). 

[17] Man, F. L. et al. The Study of Ginger-Derived Extracellular Vesicles as a Natural Nanoscale Drug Carrier and Their Intestinal Absorption in Rats. Aaps Pharmscitech 22 (2021). 

[18] Liu, Y. et al. Enhancing oral delivery of plant-derived vesicles for colitis. J Control Release357, 472-483 (2023). 

[19] Zhang, M., Wang, X., Han, M. K., Collins, J. F. & Merlin, D. Oral administration of ginger-derived nanolipids loaded with siRNA as a novel approach for efficient siRNA drug delivery to treat ulcerative colitis. Nanomedicine12, 1927-1943 (2017). 

[20] Tong, L. et al. Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota. Theranostics11, 8570-8586 (2021). 

[21] Wang, B. et al. Targeted drug delivery to intestinal macrophages by bioactive nanovesicles released from grapefruit. Mol Ther22, 522-534 (2014). 

[22] Kim, K. et al. Stability of Plant Leaf-Derived Extracellular Vesicles According to Preservative and Storage Temperature. Pharmaceutics14 (2022). 

[23] Farley, J. T., Eldahshoury, M. K. & de Marcos Lousa, C. Unconventional Secretion of Plant Extracellular Vesicles and Their Benefits to Human Health: A Mini Review. Frontiers in Cell and Developmental Biology10 (2022). 

[24] Feng, T., Wan, Y., Dai, B. & Liu, Y. Anticancer Activity of Bitter Melon-Derived Vesicles Extract against Breast Cancer. Cells12 (2023). 

[25] Cho, E. G. et al. Panax ginseng-Derived Extracellular Vesicles Facilitate Anti-Senescence Effects in Human Skin Cells: An Eco-Friendly and Sustainable Way to Use Ginseng Substances. Cells10 (2021). 

[26] Zu, M. et al. 'Green' nanotherapeutics from tea leaves for orally targeted prevention and alleviation of colon diseases. Biomaterials279, 121178 (2021). 

[27] Ju, S. et al. Grape exosome-like nanoparticles induce intestinal stem cells and protect mice from DSS-induced colitis. Mol Ther21, 1345-1357 (2013). 

[28] Deng, Z. B. et al. Broccoli-Derived Nanoparticle Inhibits Mouse Colitis by Activating Dendritic Cell AMP-Activated Protein Kinase. Molecular Therapy25, 1641-1654 (2017). 

[29] Teng, Y. et al. Plant-Derived Exosomal MicroRNAs Shape the Gut Microbiota. Cell Host Microbe24, 637-652 e638 (2018). 

[30] Chen, X. Y., Zhou, Y. & Yu, J. J. Exosome-like Nanoparticles from Ginger Rhizomes Inhibited NLRP3 Inflammasome Activation. Mol Pharmaceut16, 2690-2699 (2019). 

[31] Zhang, M. et al. Edible Ginger-derived Nano-lipids Loaded with Doxorubicin as a Novel Drug-delivery Approach for Colon Cancer Therapy. Molecular Therapy24, 1783-1796 (2016). 

[32] Zhang, M. Z., Wang, X. Y., Han, M. K., Collins, J. F. & Merlin, D. Oral administration of ginger-derived nanolipids loaded with siRNA as a novel approach for efficient siRNA drug delivery to treat ulcerative colitis. Nanomedicine12, 1927-1943 (2017).

引用本文

蔡鹏, 张明真, 高博雯, 植物源细胞外囊泡在炎症性肠病治疗中的应用[J]. 现代生物技术研究, 2023; 1: (1) : 6-11.