Study on the effect of aspirin on ACLT osteoarthritis in mice
阿司匹林抗小鼠ACLT骨性关节炎的作用研究

摘要

骨性关节炎（Osteoarthritis，OA）是由衰老、肥胖、遗传等多因素引起的常见慢性退行性疾病。阿司匹林（Aspirin）是一种具有抗炎镇痛等作用的非甾体类水杨酸药物。为探究阿司匹林对骨性关节炎的治疗作用，本研究通过切断小鼠右膝关节的前交叉韧带建立骨关节炎ACLT模型（Anterior Cruciate Ligament Transection，前交叉韧带切除术），使用25mg/kg、50mg/kg、100mg/kg的低、中、高剂量阿司匹林治疗小鼠28天，通过记录小鼠28天中的体重变化、行走距离和站立次数等评估骨性关节炎症状，28天后处死小鼠，利用HE、番红O-固绿染色观察小鼠膝关节软骨组织的变化评估阿司匹林的疗效。利用HE染色法观察小鼠内脏组织（心、肝、脾、肺和肾）的病理形态变化评估阿司匹林的潜在毒副作用。结论：小鼠骨关节炎ACLT模型造模成功，中、高剂量阿司匹林对小鼠骨关节炎有良好的缓解作用，但长时间使用过高剂量阿司匹林对小鼠的肝、肾有副作用。

Abstract

Osteoarthritis (OA) is a common chronic degenerative disease caused by aging, obesity, genetics and other factors. Aspirin is a non-steroidal salicylic acid drug with anti-inflammatory and analgesic effects. In order to explore the therapeutic effect of aspirin on osteoarthritis, the ACLT (Anterior Cruciate Ligament Transection osteoarthritis model) was established on the right knee joint of mice. After that, the mice were treated with low, medium and high doses of aspirin (i.e., 25mg/kg, 50mg/kg and 100mg/kg) for 28 days, respectively. The osteoarthritis symptoms were evaluated by recording the changes of three indicators, including body weight, walking distance and standing times of mice. To evaluate the effect of aspirin on mouse knee cartilage, HE staining and safranin O-fast green staining were used to assess changes of the mice knee cartilage tissue. To evaluate the toxicity of aspirin on visceral tissue, HE staining was used to assess for pathological morphologic changes of mouse visceral tissue.
Conclusion  : The ACLT model of mouse osteoarthritis was successfully established. Moderate and high doses of aspirin have excellent therapeutic effects on mouse osteoarthritis, but long-term use of high dose of aspirin has side effects on mouse liver and kidney.