摘要
通过在基因治疗中引入免疫激活成分的免疫基因治疗已成为治疗癌症的一种新策略。然而，目前仍然需要一个理想的平台来实现免疫激活和高效的基因传递。罗伊氏乳杆菌因其独特的抗原性具有潜在的免疫调节活性，并与癌症进展具有潜在关联。本研究通过将罗伊氏乳杆菌裂解物包封在DMP中，设计了一种新型的非病毒siRNA载体DMP-Lac。DMP-Lac能促进免疫细胞的成熟和活化，并表现出抑制肿瘤的作用。装载靶向STAT3的siRNA后,DMP-Lac/siSTAT3复合物可以有效抑制肿瘤的进展。综上所述，我们设计了一种结合免疫激活和STAT3沉默的新型中肿瘤治疗策略。
关键词： STAT3；siRNA递送；肠道益生菌；免疫基因治疗
Abstract
Immunogene therapy through the introduction of immune-activating components into gene therapy has become a new strategy for treatment of cancer. However, an ideal platform for immune activation and efficient gene delivery is still needed. Lactobacillus reuteri has potential immunomodulatory activity due to its unique antigenicity and potential association with cancer progression. In this study, a novel non-viral siRNA vector DMP-Lac was designed by encapsulating Lactobacillus reuteri lysate into DMP. DMP-Lac can promote the maturation and activation of immune cells, and has been proven to inhibit tumor. Loaded with STAT3-targeting siRNA, the DMP-Lac/siSTAT3 complex can effectively inhibit tumor progression. In summary, we have designed a novel middle tumor treatment strategy that combines immune activation and STAT3 silencing.
Key words： STAT3; siRNA delivery; intestinal probiotics; immune gene therapy
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