摘要
目的 研究μ型阿片类受体（Mu opioid receptors，MOR）高表达对血管内皮细胞增殖及迁移的影响。方法 收集颅内动脉狭窄（intracranial arterial stenosis, ICS）大鼠模型颅内狭窄动脉及侧支动脉，提取蛋白并通过Western blotting法检测MOR的表达；利用质粒高表达MOR，通过微管形成实验、集落形成实验、Transwell实验观察血管内皮细胞增殖及迁移情况。结果 Western blotting显示：与正常对照组相比，ICS大鼠侧支中MOR表达显著升高。微管形成实验显示与对照组相比，MOR高表达组显著促进了血管内皮细胞的微管形成。血管内皮细胞集落形成实验表明与对照组相比，MOR高表达组显著促进了血管内皮细胞增殖。Transwell实验证实与对照组相比，MOR高表达组显著促进了血管内皮细胞的迁移。结论 ICS大鼠的侧支动脉中MOR高表达，MOR表达升高可显著促进血管内皮细胞的增殖及迁移。
关键词： μ型阿片类受体；血管内皮细胞；颅内动脉狭窄；侧支循环
Abstract
Objectives: Study the effects of high expression of Mu opioid receptors (MOR) on the proliferation and migration of vascular endothelial cells. Methods: Collect intracranial stenotic arteries and collateral arteries from intracranial arterial stenosis (ICS) rat model, extract proteins and detect the expression of MOR through Western blotting method; use plasmid to highly express MOR, and conduct microtubule formation experiments. Colony formation assay and Transwell assay were used to observe the proliferation and migration of vascular endothelial cells. Results: Western blotting showed that MOR expression in the collateral branches of ICS rats was significantly increased compared with the normal control group. The microtubule formation experiment showed that compared with the control group, the MOR high expression group significantly promoted the formation of microtubules in vascular endothelial cells. The vascular endothelial cell colony formation experiment showed that compared with the control group, the MOR high expression group significantly promoted the proliferation of vascular endothelial cells. Transwell experiments confirmed that compared with the control group, the MOR high expression group significantly promoted the migration of vascular endothelial cells. Conclusion : MOR is highly expressed in the collateral arteries of ICS rats, and increased MOR expression can significantly promote the proliferation and migration of vascular endothelial cells.
Key words： Mu opioid receptors (MOR); vascular endothelial cells; intracranial artery stenosis; collateral circulation
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